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Articles by Iftikhar Hussain
Total Records ( 5 ) for Iftikhar Hussain
  Masood Akhtar , C.S. Hayat , M. Ashfaque and Iftikhar Hussain
  Single radial hemolysis test for the detection of antibodies against Boophilus microplus was developed. Optimum test conditions were determined on the basis of clear hemolysis and maximum haemolytic zone diameter by using human, sheep and rabbit erythrocytes; complement dilutions and temperature. There was a non significant difference (P>0.05) in average haemolytic zone diameters among the erythrocytes of three species used but qualitative analysis indicated that human group "O" erythrocytes at the rate of 2 per cent in agar gel medium gave better results. A 1:2 dilution of complement in vernal buffer at the rate of 8 percent in agar gel medium was found to be optimally appropriate. The combination of over night refrigeration and subsequent incubation at 37 °C in a humid chamber for 8-12 hours gave good results regarding the quality of the zones of hemolysis which were well delineated.
  Muhammad Javed Arshad , Muhammad Siddique and Iftikhar Hussain
  During the year 2000-2001, P. multocida isolated from natural outbreaks of fowl cholera were subjected to somatic serotyping for the first time in Pakistan. Serotyping was carried out by the gel-diffusion precipitin test. P. multocida serotype 1 predominated with the percentage of 47.62, followed by serotype 3(28.57%), 4(14.29%) and 12(09.52%). All the serotypes found highly virulent with 100% mortality after challenge by serotype 1 and 3, 80% by serotype 4 and 60% by serotype 12.
  Masood Akhtar , C.S. Hayat , M. Ashfaque , Iftikhar Hussain , Murad Ali Khan and Sultan Ayaz
  A splenic cell migration inhibition test with modifications was successfully employed in the flat bottom micro titration plates to detect the cell mediated immunity more conveniently and accurately due to vaccine against coccidiosis. A drop of melted agarose (0.2% in PBS) was used to immobilize the splenic fragments. The splenic cells were culture in Hank`s Balanced salt containing fresh chicken plasma and maintained for 10 days. Twenty four hours after cultivation, dominant cell type migrated from a splenic fragment was leukocyte-like cells. At the fourth day these appeared predominantly large round cells and resembled to the macrophage. At the 10th day, spindle cells resembling of fibroblast appeared. The migration of the splenic cells from immunized chickens were inhibited remarkably with the antigen and there was a significant difference (P<0.01) in migration distance of the splenic cells with and without the antigen.
  Mirza Ali Khan , Muhammad Ashfaque and Iftikhar Hussain
  Livers of 105 dead-in-shell embryos, randomly selected from three local hatcheries of Faisalabad were bacteriological examined. The occurrence of dead-in-shell was recorded to be 9.91 per cent (8.04 to 12.09%) positive liver samples of dead-in-shell embryos. A total of 118 bacterial isolates were obtained from the 87.62 per cent livers of dead-in shell embryos. The relative occurrence of different bacterial species were: Escherichia coli 52.54 per cent, Paratyphoid salmonellae 12.7 per cent, S. gallinarum 11.86 per cent. S. pullorum 5.93 per cent, Streptococcus faecalis 5.93 per cent, Bacillus subtilis 4.2 per cent, Pseudomonas aeruginosa 3.39 per cent and Proteus mirabilis 3.39 per cent. 28.26 per cent of positive samples yielded mixed growth of two different bacterial species and E. coli was found in all of these combinations. 91.94 per cent of the E. coli isolates were found Congo red positive. The in vitro sensitivity of the isolates to the 11 antibiotics were as: norfloxacin 100 per cent, gentamicin 89 per cent, flumequine 80 per cent, neomycin 68.64 per cent, chloramphenicol 67.80 per cent, streptopenicillin 65 per cent, erythromycin 14.4 per cent and furazolidone 13.5 per cent. Multiple drug resistance was observed among the isolates, particularly against the antibiotics frequently used in poultry.
  Masood Jan , Ghulam Hassan , Iftikhar Hussain and Razi-u-Din
  An experiment was conducted to determine the combining ability of yield and yield components in sunflower. There was a significant difference for General Combining Ability (GCA), Specific Combining Ability (SCA) and Reciprocal Effect (RE) for all the traits studied except for 100-achene weight where the GCA effect was found non-significant. In the estimates of variances, the SCA for the traits were more than GCA showing non-additive effects for all the traits, GCA: SCA ratio also indicated predominance of non-additive gene effect for these traits. Additive and non-additive contributed equally to the inheritance of the achene number plant-1 and this can easily be judged from GCA: SCA ratio. Among the inbred lines, TF-1 was the best general combiner for head size and 100-achene weight though its GCA effect was not significant for the latter. Inbred line ARI was the best general combiner for the achene yield plant-1 and achene yield kg ha-1, while TF-4 was the best combiner for achene number plant-1. Different cross combinations showed different response with respect to GCA, SCA and RE for various traits studied. Only cross combination TF-335xPESH was consistent for having high SCA for head size and achene yield kg ha-1 and TF-335xTF-11 exhibited high RE effect for achene yield plant-1 and 100-achene weight.
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