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Articles by Hossein Samadi Kafil
Total Records ( 4 ) for Hossein Samadi Kafil
  Mohammad Asgharzadeh , Saber Yousefee , Hossein Samadi Kafil , Mohammad Reza Nahaei , Khalil Ansarin and Mohammad Taghi Akhi
  To investigate the genetic variation among Mycobacterium tuberculosis isolates from East and West Azarbaijan provinces of Iran and to evaluate the manner of recent transmission of tuberculosis (TB), we performed IS6110-based restriction fragment length polymorphism analysis of isolates. Restriction fragment length polymorphism (RFLP) typing performed on 165 culture-positive specimens from East and West Azarbaijan. Using IS6110 as a probe, Mycobacterium tuberculosis strains assigned to clusters based on identical DNA fingerprints. Rates of patients have clustered were 27.68% in East and 30.19% in West Azarbaijan. There was not statistically significant differences in clustering of patients in two provinces (p = 0.4533) but infection with Mycobacterium tuberculosis in males and females in two provinces were different (p = 0.0048). In East Azarbaijan there was not difference in transmission of tuberculosis between males and females, also in males and females belonged to clusters we couldn’t find statistical difference (p = 0.1833). The rate of active transmission of TB in West Azarbaijan was slightly more than East Azarbaijan. It can be due to different factors such as poor economic and less developed condition in West Azarbaijan.
  Mohammad Asgharzadeh and Hossein Samadi Kafil
  Mannose-Binding Lectin (MBL) is a member of the collectin family. It binds to various oligosaccharides and activates the classical pathway of complement independent from C1q. The aim of present study is to study the distribution of the alleles of MBL gene and promoter variants in intracellular, extracellular pathogens and autoimmune diseases. Our studies showed occurrence of the codon 54 mutation (allele B) of MBL was associated with the occurrence of the acute hepatitis C, this showed the low MBL level can intense progress of this infection, but in other intracellular infections, low expression MBL genotypes associated with protection against these infections and wild type alleles with high MBL production considered as a risk factor for these intracellular pathogens. In extracellular pathogens, there was contrary and wild types of genotype with high production of MBL were associated with protection against these infections and alleles with low MBL production considered as a risk factor for these pathogens. In autoimmune diseases our study and other studies demonstrated that low MBL was a risk factor for these diseases.
  Mohammad Asgharzadeh , Hossein Samadi Kafil , Mohammad Ebrahim Ebrahimzadeh and Aboulfazl Bohlouli
  The aim of present study is to determine the distribution of the alleles of mannose-binding lectin gene and promoter variants in infections that cause renal dysfunctions. Fifty eight renal recipients’ samples which lost their kidneys in result of infection have compared with 120 normal controls from Azarbaijan population of Iran. Blood samples were obtained from renal transplant recipients who received renal from March 2004 to July 2005. Mannose-binding lectin genotypes have investigated by polymerase chain reaction and restriction fragment length polymorphism. Allelic and genotypic frequency of the polymorphism at position-550, +4 and at codon 52 and 57 did not show statistical differences between infected patients and controls (p>0.05) but significant frequency of allele B (codon 54) (p = 0.0011) and Ly (p = 0.007), Lx haplotype (p = 0.0002) of promoter was observed in this patients and allele A was more frequent in healthy patients. Present findings provide evidence that presence of different alleles and haplotypes that cause low concentration of mannose-binding lectin in serum is a risk factor for susceptibility to renal infections that cause renal dysfunction.
  Mohammad Asgharzadeh , Saber Yousefee , Mohammad Reza Nahaei , Mohammad Taghi Akhi , Khalil Ansarian and Hossein Samadi Kafil
  IS6110-based DNA fingerprinting is currently the most widely used genetic marker for differentiating among Mycobacterium tuberculosis strains. To evaluate the DNA polymorphism among Mycobacterium tuberculosis strains and to determine if there is matching of IS6110 fingerprints representing recent transmission of tuberculosis. Totally one hundred and sixty five isolates of M. tuberculosis (53 from West Azarbaijan and 112 from East Azarbaijan) were analyzed by IS6110 restriction fragment length polymorphism fingerprinting. Isolates having identical RFLP patterns were considered a cluster. The average number of IS6110 copies per strain was 7.3 and ranged from 0 to 17 among the M. tuberculosis isolates. The IS6110-DNA patterns from these isolates were highly polymorphic. In conclusion 123 patterns were observed which 16 patterns were shared by 47 isolates (30.52%). Most strains (93.62%) had multicopy patterns and only 3 of clustered isolates had less than six IS6110 copies. In our study increased clustering was observed with isolates from male patients. RFLP analysis of 154 isolates of M. tuberculosis showed a considerable diversity, suggesting that most patients were infected with unique strains, probably resulted from reactivation of the latent infection.
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