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Articles by H. Busby
Total Records ( 2 ) for H. Busby
  E.S. Soliman , H. Busby , C. Kilpatrick , Y. Nagamalleswari , P. McDuff and M.A.A. Sobieh
  Problem statement: Genetic line differences in resistance of layer hens and young chicks to Salmonella entritidis have been identified through a lot of studies. That is why the agricultural industry was prepared for the potential phasing out of antibiotics for use in controlling bacterial pathogens. Early infection may result in long term colonization of layers with Salmonella entritidis, resulting in shedding into table or hatching eggs. Approach: This study was carried to evaluate the genetic factors underlying early response to Salmonella entritidis, genetic line differences in mortality and pathogen load at two sites (cecal lumen and spleen) were investigated. At day of hatch, chicks of four genetic lines were intra-esophageally inoculated with one of three doses of Salmonella entritidis phage type 13 A. Results: There was a significant effect (p≤0.001) of genetic line on chick 6 days survival. The effect of genetic line was significant (p≤0.05) on survivorsí Salmonella entritidis burden in cecal content but not on Salmonella entritidis load per gram of spleen. Salmonella entritidis pathogen load of the spleen and the cecal content were not significantly correlated, indicating that independent host mechanisms are partly responsible for these two traits. Conclusion: Future Salmonella entritidis control mechanism in poultry may be the same as it is used these days but it has to be for longer term sustainability, genetic resistance should be pursued. Sufficient genetic line variation should exists to suggest that it is feasible to effectively choose among pure breeder lines for those exhibits reduced Salmonella entritidis induced mortality and cecal content Salmonella entritidis pathogen load in young layer chicks.
  S. Essam Soliman , P.G. Reddy , A.A. Mohamed Sobeih , H. Busby and E. Sara Rowe
  A total of 416 environmental samples (litter, water, swabs and air) were collected from commercial poultry farms located in Ismailia and Zagazig Governorates during the period January through July of 2008. These samples were tested by conventional cultural methods and then were confirmed biochemically. The bacterial isolates that were identified included: Citrobacter spp., E. coli, Klebsiella oxytoca, Proteus vulgaris, Pseudomonas aureuginosa, Salmonella sp, Shigella sp, Staphylococcus aureus, Streptococcus fecalis and Streptococcus pneumonie. The suspected colonies for Salmonella spp. were cultured onto a selective media (Selenite F broth and S-S agar) for further confirmation. Prevalence and frequencies of the microorganisms were calculated to detect the most predominant microorganisms. Swab samples showed higher prevalence of bacterial isolates (37.7%). Samples collected from closed house system had higher prevalence of bacterial isolates in swab samples (20.5%) as compared to samples from open house system (17.2%). Citrobacter sp (8.3%), Proteus vulgaris (8.3%) and Pseudomonas aureuginosa (16.7%) predominated in litter samples from closed house system. E. coli (35.7%) predominated in air samples of closed house system. Klebsiella oxytoca (10.0%) predominated in water of open house system. Salmonella sp (35%) predominated in swab samples of open house system. Shigella sp prevalence was similar between water samples of opened house system (6.0%) and swab samples of closed system (5.9%). Staphylococcus aureus (50.0%) predominated in air of closed house system. Streptococcus pneumonie (17.8%) predominated in air samples of open house system. Streptococcus fecalis (5.3%) predominated in litter samples of open house system. A total of 266 environmental and non-environmental samples were collected during the period September of 2008 through January of 2009 by the Alabama State Veterinary Diagnostic Laboratory as part of the National poultry improvement plan. These samples were examined using highly selective media for Salmonella sp The positive samples were confirmed biochemically and sero-grouped. The highest prevalence of Salmonella spp. was in environmental swabs (38.6%) with special reference to slat swabs (10.2%), fans (8.1%) and sills (6.9%). The highest predominant group of Salmonella spp. was C3 (50.4%) followed by group B (24.0%) and group C2 (13.9%).
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