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Articles by G.F. Dire
Total Records ( 6 ) for G.F. Dire
  G.F. Dire , J.C.S. Oliveira , S.D.D. Vasconcelos , P.R.A. Siqueira , R.M. Duarte , M.C.L. Almeida , J.S. Rodrigues , M.L. Fernandes and M. Bernardo-Filho
  The aim of this study was to assess the effect of an extract of Sechium edule on the labeling of blood elements with 99mTc. A freshly extract of Sechium edule was administered to Wistar rats during 7 days. After that, samples (0.5 mL) of blood were incubated with stannous chloride (SnCl2) and 99mTc. The blood was centrifuged and plasma (P) and RBC were isolated. P and RBC were also precipitated with trichloroacetic acid and soluble (S) and insoluble (I) fraction (F) were determined. The radioactivity (ATI%) was rated in RBC, IF-P and IF-C in a well counter. Due to the analysis of the results it was observed a decrease in the uptake of %ATI by the blood cells in the diabetic group treated with chayotte (89.96±5.16) in comparison to the diabetic group (97.16±1.26). Related to the %ATI binding in the IF-P it was noticed a decrease in the efficiency of radiolabeling in the diabetic group (from 80.22±5.50 to 71.16±1.97) and in the diabetic group treated with the extract (from 80.22±5.50 to 70.87±4.10). Due to the %ATI binding to the IF-C it was observed a decrease in the group treated with chayotte extract (from 91.08±4.10 to 67.26±2.20), in the diabetic group (from 91.08±4.10 to 71.26±2.46) and in the diabetic group treated with chayotte (from 91.08±4.10 to 71.51±3.38). In the light of the results the referred extract has an oxidant action. We suggest that the referred extract may induce the generation of activity metabolites with direct action on the labeling process probably acting in the cell membrane and in the binding sites protein together with an oxidative stress present in diabetes.
  G.F. Dire , P.R.A. Siqueira , R.M. Duarte , M.C.L. Almeida , J.S. Rodrigues , J.C.S. Oliveira , S.D.D. Vasconcelos , M.L. Fernandes and M. Bernardo-Filho
  We evaluated the influence of a chayotte(Sechium edule) extract (macerated) and diabetes status on the bioavailability of 99mTcO4Na. In this study, in the biodistribution analysis, the 99mTcO4Na (0.3 mL) was administrated into female Wistar rats (diabetes and no diabetes induced) which had drunk or not the extract (macerated) for 7 days. After 10 min, animals were sacrificed, the organs were isolated, the radioactivity determined in a well counter and the percentages of radioactivity (%ATI) in the organs was calculated. The analysis of the results has indicated an increase in the %ATI in the group treated with chayotte extract (0.0040±0.0010 to 0.0060±0.0010). In the diabetes group it was observed a decreased in the %ATI (0.0040±0.0010 to 0.0030±0.0010) as well as in the diabetes group treated with chayotte extract (0.0040±0.0010 to 0.0020±0.0003). Due to the %ATI in the muscle it was verified that there was an increase of the %ATI in the group treated with chayotte extract (0.0090±0.0040 to 0.0230±0.0070) and in the diabetes group treated with chayotte (0.0090±0.0040 to 0.0180±0.0230). It was observed a difference between the group treated with chayotte to the diabetes group (0.0230±0.0070 to 0.009±0.0040) and between the diabetes group to the diabetes group treated with chayotte extract (0.0090±0.0040 to 0.0180±0.0230). Related to %ATI in the spleen by the light of the results it was detect a difference in the uptake of sodium pertechnetate between the group treated with chayotte extract to the diabetes group treated with the referred extract (0.0290±0.0013 to 0.0480±0.0160). Due to the biodistribution it was related that an extract of eggplant was capable of altering the bioavailability of 99mTcO4Na different of a cauliflower extract which has not been able to alter it. It is possible to suggest that some components of chayotte extracts present an oxidant power able to alter the biodistribution of 99mTcO4Na, as a tip, we speculate that the referred extract when metabolized in the liver may produce reactive metabolites with oxidant properties linked to the stress which is generated by diabetic status, this fact could justify by the increase of %ATI in the brain, muscle and spleen which probably may be due to the producing of AGEs in diabetes status.
  G.F. Dire , E.A.C. Lima , M.L. Gomes , S. Moreno , M.V.C. Faria , R.L. Jales , M.T.J.A. Catanho and M. Bernardo Filho
  Natural products have been widely used by human beings. However, sometimes the biological effects of these products are not fully known. It is concerned that many natural medicines may contain potentially toxic ingredients and contaminants such as heavy metals. Red blood cells (RBC) and plasma proteins labeled with technetium-99m (99mTc) have several clinical applications and it has been reported that some natural products are capable of reducing the efficiency of this radio labeling. The aim of this work was to assess the oxidant or antioxidant action of the agents presents in the extract of Sechium edule verifying its effects on the labeling of blood elements with 99mTc and on the inhibition of the activity of acetyl cholinesterase (AchE). A freshly extract of Sechium edule was administered to Wistar rats during 15 and 60 days. After that, samples (0.5ml) of blood were incubated with stannous chloride (SnCl2) and 99mTc. The blood was centrifuged and plasma (P) and RBC were isolated. P and RBC were also precipitated with trichloroacetic acid and soluble (S) and insoluble (I) fraction (F) were determined. The results have shown that the referred extract was able to reduce the radio labeling in BC to 15 days of treatment (from 98.77%± 0.45 to 90.35%± 5.04) to 60 days of treatment (from 98.77%± 0.45 to 53.53%± 0.91), in IF-BC (from 90.36%± 2.34 to 21.20%± 2.36) and in IF-P to 15 days of treatment (77.20%± 2.19 to 53.26%± 6.69) to 60 days of treatment (77.20%± 2.19 to 11.01%±3.22). The analysis of toxicology was performed by the determination of acetyl cholinesterase activity. In the light of the results the referred extract has not shown inhibitory action due to the activity of the enzyme. In the light of the results obtained we suggest that the referred extract may induce the generation of activity metabolites with oxidant properties with direct action on the labeling process.
  G.F. Dire , S.D.D. Vasconcelos , P.R.A. Siqueira , R.M. Duarte , M.C.L. Almeida , J.S. Rodrigues , J.C.S. Oliveira , M.L.Fernandes and M. Bernardo-Filho
  The use of natural products occurs around the world. The labeling of blood constituents with technetium-99m (99m Tc) has been influenced by natural extracts and oxidative stress. Some studies suggested that maternal diabetes can affect the embryology environmental and this fact could help to elucidate that the oxidative stress may be related to the disturb of the gene expression which is essential in the control of the ontogenetic processes. We evaluated the influence of a chayotte (Sechium edule) extract on the labeling of blood elements with 99m Tc in diabetic female rats. The animals were treated with chayotte during 7 days and samples of blood were withdrawn. The samples were incubated with stannous chloride and with 99mTc. Plasma(P) and blood cells (BC) were isolated, also precipitated with trichloroacetic acid and soluble (SF) and insoluble fractions (IF) separated. The radioactivity (ATI%) was rated in RBC, IF-P and IF-C in a well counter. In the diabetic group it was observed an increase in the radioactivity in BC (from 43.65 ± 1.83 to 59.47 ± 1.83 and in the IF-BC (from 26.22 ± 23.58 to 73.28 ± 23.58). It was noticed that the referred extract has normalizing the efficiency of radiolabeling in the diabetic animals which have received the referred extract. The effect of chayotte extract probably, could be explained by the metabolization of the chayotte that would be capable of inducing the generation of active metabolites with oxidant properties probably altering the activity of cell membrane.
  G.F. Dire , M.F.M.C. Coura , M.C.L. Almeida , S.D.D. Vasconcelos , P.R.A. Siqueira , R.M. Duarte , J.S. Rodrigues , J.C.S. Oliveira , M.L. Fernandes and M. Bernardo-Filho
  Stannous chloride (SnCl2) is employed as a reducing agent to obtain Technetium-99m-labelled radiopharmaceuticals in nuclear medicine kits, being inject endogenously in humans. Toxic effects of. these kits were not studied, thus making it important to evaluate their impact in humans. The use of natural extracts as medicines is growing around the world. The chayotte (Sechium edule) is a subtropical vegetable with potent diuretic action. It is used in folk medicine due its hypotensor effect. In this study, plasmid deoxyribonucleic acid (DNA) was exposed to chayotte extracts (macerated) (0.1g mL-1) in presence of stannous chloride (SnCl2). Samples of the plasmid DNA were analyzed through agarose gel electrophoresis. The results show that the chayotte extract was capable of damaging the DNA in the presence and in the absent of SnCl2.
  D.P.C. Costa , A.P.S. Cruz , L.L.F. Aguiar , J.C.S. Oliveira , G.L.T. Fernandes , S.D.D. Vasconcelos , J.S. Rodrigues , G.F. Dire and H.R. Borba
  This approach intends to add new data on the helminthes parasites of laboratory mice. It has been investigated the anthelmintic activity of Solanum lycocarpum (Solanaceae) extracts against Vampirolepis nana and Aspiculuris tetraptera in mice naturally infected. The extracts were applied for oral saw (intragastric), into the volume of 0.04 mL g-1, with the employing of a dead and bend probe during three consecutive days. The fecal material, collected 24 h after each application, performing a total of four fecal collection, have been softened previously, transferred about to sieve of network of 125 µm and tested under microscope stereoscope, with the objective of behave the identification and counting from the worms eliminated of the second to the fifth day of the experimental. Tukey-Kramer Multiple Comparisons Test was applied to compare the results. According to the analysis of the results it was observed that there were no differences in the % of elimination to the concentration of 10% between TM and UR (from 28.4±3.55 to 29.2±2.92) and statistical differences between TM and C (from 28.4±3.55 to 15.0±1.5) and UR and C (from 29.2±2.92 to 15.0±1.5). To the concentration of was observed that there were differences in the of eliminating between TM and UR (from 30.9±3.43 to 7.7±1.1) and C (from 30.9±3.43 to 15±1.5) related to the experimental which the animal was infecting with Vampirolepis nana. To the concentration of 5% it was observed that there were no differences in the % of elimination between TM and UR (from 4.18±3.33 to 3.70±3.16), TM and C (from 4.18±3.33 to 1.56±3.16) and UR and C (from 3.70±3.16 to 1.56±3.16) related to the experimental which the animal was infected with Aspiculuris tetraptera. It was described that in the chemical analysis of the studied extract there is the presence of glycoalkaloids, a finding that represents a reason for concern since many of these substances are generally toxic and maybe anthelmintic. It was published that medicinal plants which were reported as useful in the treatment of diabetes the S. lycocarpum was the sixth most frequently mentioned. According to the results obtained in the present study, we can speculate that the anthelmintic effect of S. lycocarpum would be related to the action of steroidal alkaloid oligoglycosides as well as the presence of short-chain fatty acids as well as with the concentration of the referred compounds in the extracts.
 
 
 
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