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Articles by Francisco Suarez Guemes
Total Records ( 2 ) for Francisco Suarez Guemes
  Jorge Acosta Dibarrat , Efren Diaz Aparicio , Victor Ruben Tenorio Gutierrez , Francisco Suarez Guemes and Jorge Tortora Perez
  In experimentally infected rams with Actiobacillus seminis, pathological changes (reproductive organs) and antibodies (IgM, IgG and IgA) in serum, seminal plasma and smegma were evaluated. Twelve 1-year old rams were infected via intraurethal IU group (n = 4) and intraepididymal IE group (n = 4) with A. seminis; four animals were kept as Controls (CON) and inoculated both ways (intraepididymal and intraurethal) with saline solution. Blood, seminal plasma and smegma were collected, during 9 weeks: 4 before and 5 after inoculation. Bacteriological analysis of semen was performed. Animals were euthanized 35 days postinoculation; reproductive organs were collected for bacteriological and histopathological analysis. IE animals developed epididymitis. Ampullae and seminal vesicles in the IE and IU showed interstitial lymphoplasmacytic infiltrates. Thus, the bacteria were not re-isolated. Two controls developed epididymal granuloma. A. seminis was re-isolated in most of the semen samples and reproductive organ of IE animals, but not in those of the IU. Postinoculation mean IgG titer were different between CON and IE in serum weeks 1 to 5 (p <0.01), seminal plasma 1, 3, 4 and 5 weeks (p <0.05) and smegma 2, 3, 4 weeks (p <0.05), likewise between the IU and CON in serum IgG titers in the 2, 3 and 5 weeks (p<0.05). Postinculation mean IgM titers were different between CON and IE in the first week (p<0.05). No differences were observed in the IgA means. Serological response (IgG/IgM) and. pathological changes (reproductive organs) were induced by IE and IU inoculation of A. seminis.
  Marisela Leal-Hernandez , Enrique Salas Tellez , Alma Nunez Del Arco , Rodolfo A. Ugalde , Victor R. Tenorio-Gutierrez , Rigoberto Hernandez-Castro , Francisco Suarez Guemes and Efren Diaz-Aparicio
  The aim of this research was to evaluate the humoral and cellular response, produced in mice inoculated with Brucella ovis and mutants in virB10 and virB11, measuring IgG1, IgG2a, IgG2b and IgM levels and cytokines production IL-2, IL-4 e IFN-γ, in lymphocyte culture. Mice of 6-weeks-old Balb/c were immunized intraperitoneally with each strain of B. ovis and the mutants with 3x1013 CFU mL-1 in PBS. For the cellular response, they were injected 2 times. In the humoral response the predominant antibodies were IgG2b and IgM in the different strains. The cytokine production with the wild type were indicative of Th2 and low response of IFN-γ and IL-2, that is indicative of an answer Th1 type characteristic of intracellular bacteria. The Reo 198 strain, showed Th1 type response. The mutant ΔvirB11 only presented response to IFN-γ 24 h that decreases after 48 h and with the mutant virB10:: Gm the shown levels of the different cytokine were low, this is indicative that the genes virB10 and virB11 of B. ovis need are complete to induce a suitable immune response. In the virulence in mice only recovered the Reo and wild type strains until 1 week postinoculation and the mutants can recover until 4 and 5 weeks postinoculation. One concludes the immune response by the different strains of B. ovis was moderate and can be attributed to that the murine model not was the more recommended to evaluate to cellular response would presumably have been specific to specie by the poorly or inability response showed when were mice inoculated with the different strains of B. ovis.
 
 
 
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