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Journal of Animal and Veterinary Advances
Year: 2009  |  Volume: 8  |  Issue: 5  |  Page No.: 928 - 934

Immune Response Produced by Brucella ovis and Mutants in virB10 and virB11 in Murine Model

Marisela Leal-Hernandez, Enrique Salas Tellez, Alma Nunez Del Arco, Rodolfo A. Ugalde, Victor R. Tenorio-Gutierrez, Rigoberto Hernandez-Castro, Francisco Suarez Guemes and Efren Diaz-Aparicio    

Abstract: The aim of this research was to evaluate the humoral and cellular response, produced in mice inoculated with Brucella ovis and mutants in virB10 and virB11, measuring IgG1, IgG2a, IgG2b and IgM levels and cytokines production IL-2, IL-4 e IFN-γ, in lymphocyte culture. Mice of 6-weeks-old Balb/c were immunized intraperitoneally with each strain of B. ovis and the mutants with 3x1013 CFU mL-1 in PBS. For the cellular response, they were injected 2 times. In the humoral response the predominant antibodies were IgG2b and IgM in the different strains. The cytokine production with the wild type were indicative of Th2 and low response of IFN-γ and IL-2, that is indicative of an answer Th1 type characteristic of intracellular bacteria. The Reo 198 strain, showed Th1 type response. The mutant ΔvirB11 only presented response to IFN-γ 24 h that decreases after 48 h and with the mutant virB10:: Gm the shown levels of the different cytokine were low, this is indicative that the genes virB10 and virB11 of B. ovis need are complete to induce a suitable immune response. In the virulence in mice only recovered the Reo and wild type strains until 1 week postinoculation and the mutants can recover until 4 and 5 weeks postinoculation. One concludes the immune response by the different strains of B. ovis was moderate and can be attributed to that the murine model not was the more recommended to evaluate to cellular response would presumably have been specific to specie by the poorly or inability response showed when were mice inoculated with the different strains of B. ovis.

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