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Articles by Fazal Hadi
Total Records ( 2 ) for Fazal Hadi
  Muhammad Amir Zia , Fazal Hadi , Haji Akbar , Fazal Akbar , Zakir Ullah and Israr Khan
  This research was conducted to characterize B. napus seeds and to compare seeds of different varieties for their compositions. The seeds of ten different Brassica napus varieties namely Cyclon, Star, Vangard, MLCP-048, Bullet, Altex, Con-III, Deffender, Hyola and Dunkled were analyzed for parameters like, pH, electrical conductivity and thousand seed mass. Minerals (Na+ and K+), vitamin C and protein contents were also analyzed. Na+ and K+ were determined by Flame photometer and proteins were analyzed by SDS-PAGE (Sodium dodecyl sulfate polyacrylamide gel electrophoresis). The amount of sodium ranges from 0.020-0.039%. The lowest value of sodium was found in Vangard and the highest in Cyclone. While potassium ranges from 1.14-1.42% with the lowest value for Bullet and the highest for Star. Vitamin C was found in all the varieties in range of 30-96 mg/100 g. The maximum amount of vitamin C was found in Bullet variety while minimum concentration in MLCP-048. Maximum value of pH (6.933) was recorded for MLCP-048, while minimum value for Altex 6.632. The thousand seed mass for cyclone was 5.52 g that was maximum and the lowest value was for bullet variety (3.63 g). Electrical conductivity for different varieties was estimated after different time intervals (20, 40, 60, 80, 100 and 120 min). The overall electrical conductivity determined by conductometer ranged from 0.9-10.4 μ sec. Seeds proteins were analyzed by SDS-PAGE and different numbers of bands showed that variation existed among the different varieties.
  Muhammad Tariq , Gowher Ali , Fazal Hadi , Shakeel Ahmad , Nasir Ali and Aftab Ali Shah
 

The objective of the present study was to develop an effective protocol for optimum callus induction and complete plant regeneration for four varieties of rice (Oryza sativa L.) i.e., Super Basmati, Basmati-370, Basmati-371 and Fakhre Malakand. Calli were induced from mature seed scutelum. The Murashige and Skoog (MS) and Chu`s N6 media containing hormone 2, 4-D (2, 4-Dichlorophenoxy acetic acid) in different concentrations were used for callus induction. Fakhre Malakand produced maximum calli on N6 media containing 3 mg L-1 2,4-D. while other three varieties showed maximum callus induction on N6 media containing 2.5 mg L-1 2,4-D. N6 media was found better than MS media for callus induction. For complete plant regeneration the calli of two varieties i.e., Basmati-370 and Basmati-371 were plated on N6 media containing different concentrations of NAA (1-Naphthalene acetic acid) and BAP (6-benzyl aminopurine). The maximum regeneration frequency (%) was observed on N6 media containing NAA 1 mg L-1 and BAP 2.5 mg L-1. It took 27-30 days for the callus to regenerate into a complete plant. Basmati-370 produced 4-7 plantlets per callus whereas Basmati-371 produced 4-8 plantlets per callus with regeneration frequencies of 61 and 69%, respectively.

 
 
 
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