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Asian Journal of Plant Sciences
Year: 2008  |  Volume: 7  |  Issue: 1  |  Page No.: 85 - 89

Physiochemical and Molecular Analysis of Brassica napus Seeds of Different Varieties

Muhammad Amir Zia, Fazal Hadi, Haji Akbar, Fazal Akbar, Zakir Ullah and Israr Khan    

Abstract: This research was conducted to characterize B. napus seeds and to compare seeds of different varieties for their compositions. The seeds of ten different Brassica napus varieties namely Cyclon, Star, Vangard, MLCP-048, Bullet, Altex, Con-III, Deffender, Hyola and Dunkled were analyzed for parameters like, pH, electrical conductivity and thousand seed mass. Minerals (Na+ and K+), vitamin C and protein contents were also analyzed. Na+ and K+ were determined by Flame photometer and proteins were analyzed by SDS-PAGE (Sodium dodecyl sulfate polyacrylamide gel electrophoresis). The amount of sodium ranges from 0.020-0.039%. The lowest value of sodium was found in Vangard and the highest in Cyclone. While potassium ranges from 1.14-1.42% with the lowest value for Bullet and the highest for Star. Vitamin C was found in all the varieties in range of 30-96 mg/100 g. The maximum amount of vitamin C was found in Bullet variety while minimum concentration in MLCP-048. Maximum value of pH (6.933) was recorded for MLCP-048, while minimum value for Altex 6.632. The thousand seed mass for cyclone was 5.52 g that was maximum and the lowest value was for bullet variety (3.63 g). Electrical conductivity for different varieties was estimated after different time intervals (20, 40, 60, 80, 100 and 120 min). The overall electrical conductivity determined by conductometer ranged from 0.9-10.4 μ sec. Seeds proteins were analyzed by SDS-PAGE and different numbers of bands showed that variation existed among the different varieties.

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