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Articles by Chao-Lin Kuo
Total Records ( 3 ) for Chao-Lin Kuo
  Hung-Chi Chang , Chia-Yung Lu , Chia-Chen Chen , Chao-Lin Kuo , Hsin-Sheng Tsay and Dinesh Chandra Agrawal
  Background and Objective: Drosera spatulata ‘Sundew’ is a carnivorous plant valued for its pharmaceutical properties. Indiscriminate use of D. spatulata by herbal industries has made the species highly vulnerable and resulted in the dwindling wild population. Therefore, the aim of the present study was to develop a simple micro propagation system and to carry out HPLC analysis of plumbagin in tissue cultured plants and in vitro shoots. Materials and Methods: Leaf explants from previously grown aseptic shoots were cultured on ½X Murashige and Skoog’s (MS) salts and vitamins (MS basal medium) supplemented with 6-benzyladenine (0.1-2.0 mg L–1) or kinetin (0.1-2.0 mg L–1), 3% sucrose and 0.35% gelrite. For rooting, four different basal media, i.e., B5, N6, WPM and Murashige and Skoog were tested. Finally, HPLC analysis of plumbagin in tissue cultured plants and commercial crude drug samples was carried out. Results: The maximum adventitious shoots (6.4/leaf explant) could be induced on ½X MS basal medium supplemented with kinetin (0.1 mg L–1), 3% sucrose and 0.35% gelrite. Induction of rooting in cent percent in vitro shoots with an average number of 48.9 roots was achieved on ½X MS basal medium without any growth regulator. Out of 10 potting mix tested, a mixture of sphagnum: scrap of cyatheaceae (1:1, v/v) was found to be the most suitable with a plant survival rate of 90%. HPLC analysis results showed that in vitro derived greenhouse plants contained plumbagin comparable to a commercial crude drug. Conclusion: The study demonstrates the production of precious plumbagin, a plant derived naphthoquinone in tissue cultures of Drosera spatulata species.
  Yong-Ren Pu , Su-Hsing Lee and Chao-Lin Kuo
  Quantitative information collection is important for scholars using questionnaires, since the number of administered questionnaires in a major survey is usually quite large. To develop an automatic and vast input method for questionnaires by computer vision, the authors adopt the perspective projection model in the image processing. This study presents a method to calibrate the camera used in the framework of this questionnaire input system. The distortions of the questionnaire image are derived therein so that the system is able to locate all checkboxes accurately. The computer software is coded with a visual programming language and is developed to extract the interiors of all checkboxes by seeking their edges. The experiment is designed, by reducing the errors produced by the recognized centers of some designated checkboxes, to determine the optimal values of various focal lengths to the image plane.
  Chao-Lin Kuo , Cheng-Chi Wang and Neng-Sheng Pai
  This study introduces a variable structure technology for the synchronization of chaos between two different hyperchaotic systems with input nonlinearity. Based on Lyapunov stability theory, a sliding mode controller and some generic sufficient conditions for global asymptotic synchronization are designed such that the error dynamics of the hyperchaotic Rössler and hyperchaotic Chen systems satisfy stability in the Lyapunov sense in spit of the input nonlinearity. The numerical simulation results demonstrate the validity and feasibility of the proposed controller.
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