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Year: 2019  |  Volume: 18  |  Issue: 1  |  Page No.: 24 - 31

Plumbagin, a Plant-derived Naphthoquinone Production in Tissue Cultures of Drosera spatulata Labill

Hung-Chi Chang, Chia-Yung Lu, Chia-Chen Chen, Chao-Lin Kuo, Hsin-Sheng Tsay and Dinesh Chandra Agrawal    

Abstract: Background and Objective: Drosera spatulata ‘Sundew’ is a carnivorous plant valued for its pharmaceutical properties. Indiscriminate use of D. spatulata by herbal industries has made the species highly vulnerable and resulted in the dwindling wild population. Therefore, the aim of the present study was to develop a simple micro propagation system and to carry out HPLC analysis of plumbagin in tissue cultured plants and in vitro shoots. Materials and Methods: Leaf explants from previously grown aseptic shoots were cultured on ½X Murashige and Skoog’s (MS) salts and vitamins (MS basal medium) supplemented with 6-benzyladenine (0.1-2.0 mg L–1) or kinetin (0.1-2.0 mg L–1), 3% sucrose and 0.35% gelrite. For rooting, four different basal media, i.e., B5, N6, WPM and Murashige and Skoog were tested. Finally, HPLC analysis of plumbagin in tissue cultured plants and commercial crude drug samples was carried out. Results: The maximum adventitious shoots (6.4/leaf explant) could be induced on ½X MS basal medium supplemented with kinetin (0.1 mg L–1), 3% sucrose and 0.35% gelrite. Induction of rooting in cent percent in vitro shoots with an average number of 48.9 roots was achieved on ½X MS basal medium without any growth regulator. Out of 10 potting mix tested, a mixture of sphagnum: scrap of cyatheaceae (1:1, v/v) was found to be the most suitable with a plant survival rate of 90%. HPLC analysis results showed that in vitro derived greenhouse plants contained plumbagin comparable to a commercial crude drug. Conclusion: The study demonstrates the production of precious plumbagin, a plant derived naphthoquinone in tissue cultures of Drosera spatulata species.

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