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Articles by C.S. Hayat
Total Records ( 3 ) for C.S. Hayat
  Masood Akhtar and C.S. Hayat
  Optimum conditions for erythrocyte rosette formation (ERF) assay were determined to detect the cellular responses in immunized buffaloes against ticks. ERF with buffalo lymphocytes was maximum at 30°C for 30 minutes. The gentle handling of cell mixtures (lymphocytes and RBC) was recommended since binding forces between red cells and T-lymphocytes are week and easily disrupted. It was found that the buffalo lymphocytes did not bind to rabbit erythrocytes to form rosettes. The appearance of rosettes formed with sheep RBC was indistinguishable in appearance from rosettes formed with human erythrocytes. The percentage of E-rosettes formed with sheep RBC was slightly less (p>0.05) (mean 28.00±5.8, range 19- 38), than the percentage formed with human RBC (mean 31.90+7.7, range 17-45). The test was found to be quick, convenient and sensitive to detect the cellular responses in immunized buffaloes against ticks.
  M. Anwar and C.S. Hayat
  Camel guts (n =600) were examined to determine the gastrointestinal parasitic fauna. The overall prevalence was recorded as 69.1 (415/600) percent. The prevalence of nematodes, cestodes and trematodes was 42.3, 22.5 and 4.3 percent, respectively. The infected guts (n = 415) had a mixed infection (32.5%) of different species of nematodes viz., Haemonchus contortus, Trichuris ovis, Trichuris globulosa, Trichostrongylus probolurus, Camelostrongylus mentulatus, Ostertagia circumcincta, Chabertia ovine, Oesophagostomum venulosum and Cooperia onchophora. The species of cestodes identified included Moniezia expansa, Moniezia benedni, Stilesia globipunctata and those of trematodes Paramphistomum cervi, Carmyerious spatiosus and Gastrothylax crumenifer, The coprological examination revealed three protozoan species i.e. Eimena cameli, Eimena (Globidium) cameli and Eimeria clromedani.
  Masood Akhtar , C.S. Hayat , M. Ashfaque , I. Hussain , Murad Ali Khan and Sultan Ayaz
  Delayed hypersensitivity reaction was used to measure the cell mediated immunity against avian coccidiosis. There was a gradual increase in the comb thickness of the immunized birds of all the groups from 24 to 72 hours after intradermal injection. The mean difference in comb thickness before and after injection of the respective antigen for the group immunized with Vaccine I (sonicated supernatant) was 1.87 mm at 24 hours, 2.23 mm at 36 hours, 2.31 mm at 48 hours and 2.42 mm at 72 hours. There was a significant difference (p<0.01) in comb thickness at 24 and 36 hours after intradermal injection. The mean difference in comb thickness before and after injection of the respective antigen for the group immunized with Vaccine II (sonicated sediment) was 1.12 mm at 24 hours, 1.75 mm at 36 hours, 2.10 mm at 48 hours and 2.31 mm at 72 hours. The difference was significant (p<0.01) at 24 and 48 hours; 36 and 72 hours after injection. Maximum difference in comb thickness was recorded 72 hours after injection in birds immunized with Vaccine I. It appears that delayed hypersensitivity response to sonicated supernatant antigen reported herein represent a strong CMI reaction. Delayed hypersensitivity comb reaction is a quick, simple, economical and practical tool to routinely determine the immune status of a bird with out restoring to challenge.
 
 
 
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