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Pakistan Journal of Biological Sciences
Year: 1998  |  Volume: 1  |  Issue: 4  |  Page No.: 383 - 385

Erythrocyte Rosette Formation Assay for the Detection of Cell Mediated Immune Responses in Buffaloes Vaccinated Against Ticks

Masood Akhtar and C.S. Hayat    

Abstract: Optimum conditions for erythrocyte rosette formation (ERF) assay were determined to detect the cellular responses in immunized buffaloes against ticks. ERF with buffalo lymphocytes was maximum at 30°C for 30 minutes. The gentle handling of cell mixtures (lymphocytes and RBC) was recommended since binding forces between red cells and T-lymphocytes are week and easily disrupted. It was found that the buffalo lymphocytes did not bind to rabbit erythrocytes to form rosettes. The appearance of rosettes formed with sheep RBC was indistinguishable in appearance from rosettes formed with human erythrocytes. The percentage of E-rosettes formed with sheep RBC was slightly less (p>0.05) (mean 28.00±5.8, range 19- 38), than the percentage formed with human RBC (mean 31.90+7.7, range 17-45). The test was found to be quick, convenient and sensitive to detect the cellular responses in immunized buffaloes against ticks.

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