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Articles by Ashwani Kumar
Total Records ( 5 ) for Ashwani Kumar
  Manorma Sharma , Archana Sharma , Ashwani Kumar and Saikat Kumar Basu
  Planttissue culturesare exposed to stresses and stress combinations that they may not have encountered in nature in their long evolution. It is a remarkable reflection on the plasticity of the plant genome that it can decipher and respond to novel in vitro stresses. Today various tissue culture techniques are used to enhance yield of secondary metabolites by trigger stress response like using Elicitors, Precursors and Biotransformation, change in environmental conditions, change in medium constituents etc. The focus of the study is to enhancement of secondary metabolites by triggerthe expression of stress marker genes. This would help in conservation of this species and provide pharmaceutical component in less time and cheap cost.
  R.B. Singandhupe , J. Patnaik and Ashwani Kumar
  The major objectives of the project is to assess lithology of bore wells, impact of canal irrigation water on ground water regimes, water quality of return flow and bore well/plezameters wells and assessment of crop water demand and supply during crop season. The lithological data of 27 bore well/piezometer showed that top layer up to 10 m depth is brown to light brown in colour and crystalline in texture. Beyond this depth it is highly weathered rock, hard rocks and granite and the colour is grey to deep grey. Such type of features resulted in to very low water discharge rate i.e 0.16 to 1.62 l/sec within 35 to 45 m bore well depth. On an average of 27 bore wells, pH of bore well water was 8.25 in pre-monsoon and 8.30 in post monsoon period. Overall the quality of irrigation water was good and was suitable for irrigation as well as domestic use. The concentration of total alkalinity, hardness, calcium, magnesium increased in post monsoon period over pre-monsoon period but reverse was the trend with carbonate concentration. High and positive correlation of pH with carbonate was found. Correlation coefficient of total hardness with calcium, magnesium, chloride was positive and highly significant. Similarly very high correlation of alkalinity with calcium and magnesium indicated higher concentration of its carbonate and bicarbonate salts in ground water.
  H.K. Aggarwal , Deepak Jain and Ashwani Kumar
  Secondary hyperparathyroidism is most common complication arising in chronic kidney disease as a part of mineral bone disorder. Doxercalciferol effectively suppresses parathyroid hormone level without having side effects like hypercalcemia and hyperphosphatemia, which are much common with earlier vitamin D analogues. It was an open, prospective study of three months duration conducted on fifty adult patients of chronic kidney disease stage III or IV already on regular follow up of kidney and dialysis clinic at Pt. B.D. Sharma PGIMS, Rohtak. Patients were randomly assigned to one of the two groups, Group I (n = 25) patients received calcitriol in dosage of 0.5 μg day-1 and Group II (n = 25) received doxercalciferol in dosage of 1 μg day-1. Patients were followed and renal investigations were carried out on monthly basis, iPTH levels were measured in the beginning and at the end of the study. The mean iPTH level decreased by 13.44% in group I and 43.1% in group II after 12 weeks of treatment (p<0.05). There were less episodes of hypercalcemia with doxercalciferol (3 patients) as compared to calcitriol (8 patients). No statistically significant difference was observed in serum phosphorous, serum calcium phosphate product and GFR in both the groups. Doxercalciferol is more effective than calcitriol in controlling secondary hyperparathyroidism in patients of CKD without any adverse effects like hypercalcemia.
  Manorma Sharma , Archana Sharma and Ashwani Kumar
  The aim of present study was to evaluate immunomodulatory potential of ethanolic extract of Asparagus racemosus Wild. and Chlorophytum arundinaceum Baker. in comparison to established immunosuppressant Cedrus deodara wood oil (100 mg kg-1 b.wt.) in mice. The assessment of immunomodulatory activity on specific and nonspecific immunity was studied by Delayed Type Hypersensitivity (DTH) and neutrophil adhesion test respectively. Through Neutrophil adhesion test maximum sensitivity was observed in in vivo extract of C. arundinaceum while percent inhibition was maximum in in vitro extract of A. racemosus. In vivo Chlorophytum arundinaceum extract at 300 mg kg-1 dose statistically significantly inhibit the adhesion of neutrophils to nylon fiber. However, in vivo A. racemosus at the dose of 300 mg kg-1 was found to significantly decreased rat paw edema as compared to control, in vivo C. arundinaceumand in vitro A. racemosus extract. The present study has shown the immunomodulatory activity of both plants by potentiating humoral as well as cellular immunity.
  Ashwani Kumar , Jessy S. Deshane , David K. Crossman , Subhashini Bolisetty , Bo-Shiun Yan , Igor Kramnik , Anupam Agarwal and Adrie J. C. Steyn
  The mechanisms that allow Mycobacterium tuberculosis (Mtb) to persist in human tissue for decades and to then abruptly cause disease are not clearly understood. Regulatory elements thought to assist Mtb to enter such a state include the heme two-component sensor kinases DosS and DosT and the cognate response regulator DosR. We have demonstrated previously that O2, nitric oxide (NO), and carbon monoxide (CO) are regulatory ligands of DosS and DosT. Here, we show that in addition to O2 and NO, CO induces the complete Mtb dormancy (Dos) regulon. Notably, we demonstrate that CO is primarily sensed through DosS to induce the Dos regulon, whereas DosT plays a less prominent role. We also show that Mtb infection of macrophage cells significantly increases the expression, protein levels, and enzymatic activity of heme oxygenase-1 (HO-1, the enzyme that produces CO), in an NO-independent manner. Furthermore, exploiting HO-1+/+ and HO-1-/- bone marrow-derived macrophages, we demonstrate that physiologically relevant levels of CO induce the Dos regulon. Finally, we demonstrate that increased HO-1 mRNA and protein levels are produced in the lungs of Mtb-infected mice. Our data suggest that during infection, O2, NO, and CO are being sensed concurrently rather than independently via DosS and DosT. We conclude that CO, a previously unrecognized host factor, is a physiologically relevant Mtb signal capable of inducing the Dos regulon, which introduces a new paradigm for understanding the molecular basis of Mtb persistence.
 
 
 
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