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Articles by Ashish Srivastava
Total Records ( 2 ) for Ashish Srivastava
  Deepti Singh , Amit Kumar Verma , Amit Kumar , Mukesh Srivastava , Shanker Kumar Singh , Arvind Kumar Tripathi , Ashish Srivastava and Iftekhar Ahmed
  The early detection of the Canine Parvo Virus (CPV) is of paramount importance. The present study was aimed to know the molecular epidemiology of Canine parvo virus. Canine faecal samples from 100 dogs showing the clinical signs of gastroenteritis in and around Mathura, Uttar Pradesh, India were collected and DNA was extracted by phenol-chloroform method. CPV vaccine strain was used as a positive control. Polymerase Chain Reaction (PCR) was carried out to amplify VPI/VP2 gene using a set of 20-mer primers [pCPV-RT (Forward): 5’-CAT TGG GCT TAC CAC CAT TT-3’; (Reverse): 5’-CCA ACC TCA GCT GGT CTC AT-3’)] from position 3136-3155 to 3276-3295 of VP1/VP2 gene. A PCR product of approximately 160 bp was generated with positive faecal samples and CPV vaccine strain. After screening, 63 dogs were found positive for CPV but no sex variation was noticed amongst the CPV positive cases. Dogs, of the age group of ≤6 months were more susceptible in comparison to of >6 months and highest occurrence was noted in unvaccinated dogs and dogs in co-habitation with other dogs. Breed wise distribution of CPV in dogs revealed that the prevalence of CPV was the highest in Doberman (77.78%), followed by Spitz (78.57%), German shepherd (70.00%), Labrador (68.75%), Pomeranian (45.45%). It is concluded that CPV is prevalent in the Mathura and nearby area and it is more common in pups of age less than 6 months old and more prevalent in German shepherd, Labrador and Pomeranian breeds of dog.
  Mukesh Srivastava , Rajeev Vasantrao Gaikwad , Abdul Samad , Barkha Sharma and Ashish Srivastava
  Glomerular Filtration Rate (GFR) is considered as the best single parameter for assessing renal function because it is directly proportional to the number of functioning nephrons. Serum creatinine is the most frequently measured analyze in clinical biochemistry as an indirect indicator of glomerular Filtration Rate (GFR). Renal scintigraphy has been used as direct measurement of the glomerular Filtration Rate (GFR) either of individual kidney or global (both kidneys). By this technique, the GFR is calculated by a regression equation relating the percent of the injected dose of radiopharmaceutical, technetium-99m Diethylene-Triaminepentaacetic acid (DTPA), taken up and filtered by the kidneys. This communication reports the relationship of direct (scintigraphy) and indirect (creatinine) methods of GFR measurement. Scintigraphy is a quick, noninvasive diagnostic technique in which a two-dimensional picture of internal body tissue is produced through the detection of the gamma radiation emitted by radioactive substances injected into the body within 15 min with a gamma camera. Serum creatinine increases in renal failure correlating with a decrease in GFR forming a curvilinear relationship. Based on the study undertaken in 44 dogs with renal failure, it was found that 86.36% dogs suffering from renal failure showed a conventional pattern of curvilinear relationship between creatinine and GFR. Inter-individual variations were observed in 11.36% of dogs. False positive results were obtained in 9% of total dogs, where creatinine was within normal reference range but GFR decreased on scintigraphic analysis. False negative observations were seen in 4.5% of dogs, where normal GFR with marginally high creatinine values was reported.
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