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Articles by A. Habib
Total Records ( 3 ) for A. Habib
  M.M. Rahman , M.N. Amin , T. Ahamed , M. R. Ali and A. Habib
  Somatic embryogenesis and subsequent plantlets regeneration were achieved in callus cultures established from the leaf base explants of Kaempferia galanga L. Callus induction and somatic embryogenesis at various frequencies were observed using different concentrations and combinations of growth regulators. The highest percentage of callus induction was observed on MS medium supplemented with 1.5 mg L-1 2,4-D+1.0 mg L-1 BA. After transfer on MS medium supplemented with 2.0 mg L-1 BA+0.1 mg L-1 NAA, this callus produced small globular embryos first that later developed into plantlets by further subculturing on the same medium. Plantlets were acclimatized and subsequently transferred to the field. Survival rate of the plantlets under ex vitro condition was 85%.
  A. Habib , M.R. Ali , M.N. Amin and M.M. Rahman
  The present study was undertaken with a view to develop a reproducible protocol for in vitro propagation of white mulberry (Morus alba L.). For this purpose, surface sterilization of the explants from field grown mature plants was done with 0.1% HgCl2 with a treatment duration of 5 minutes for shoot tip and that with 6 minutes for nodal segments. Between the two explants used, nodal explant exhibit comparatively better response to axillary shoot proliferation. Analysis of the results obtained from the proliferation experiment proved that effectiveness of cytokinin BA was better than that of Kn with respect to axillary shoot formation. And that MS medium supplemented with 1.0 mg l G 1 BA was found to be best where 100% of the explants proliferated with the axillary shoots having average length of 5.46 " 0.02 cm and a multiplication rate of 7 fold per 4-week. On the other hand, percentage of root induction and number of roots per shoot were largely affected by the concentration of MS medium and type of the auxins used. The highest percentage (100%) of root regeneration was obtained in half MS medium supplemented with 0.5% mg l G 1 IBA.
  M.M. Rahman , M.N. Amin , T. Ahamed , S. Ahmad , A. Habib , R. Ahmed , M.B. Ahmed and M.R. Ali
  An efficient protocol has been established for rapid production of plantlets using rhizome tip and lateral bud explants of the field grown plant. The explants were cultured on MS medium with auxins (NAA, IBA and IAA) and cytokinins (BA and Kn). Cent percent of the explants produced two or three shoot buds in each culture when they were cultured on MS medium containing 1.0 mg L -1 BA+0.1 mg L -1 NAA within three weeks of culture. The number of shoots per culture increased gradually when the primary cultures were subcultured in two weeks intervals. Highest number of 20.50±1.80 shoots proliferated in each culture when the explants of initially sprouted shoots were subcultured at three times on the same medium. Microshoots were isolated from the in vitro proliferated cluster of shoots produced roots in 100% cases on modified (MMS2) medium supplemented with 0.2 mg L -1 of IBA. Maximum number of 12.4±1.23 roots per microshoot were recorded on the medium containing 0.2 mg L -1 IBA. The regenerated plantlets were acclimatized and established on the soil with eighty five percent success.
 
 
 
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