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Asian Journal of Plant Sciences

Year: 2004  |  Volume: 3  |  Issue: 6  |  Page No.: 675 - 678

Efficient Plant Regeneration Through Somatic Embryogenesis from Leaf Base-derived Callus of Kaempferia galanga L.

M.M. Rahman, M.N. Amin, T. Ahamed, M. R. Ali and A. Habib


Somatic embryogenesis and subsequent plantlets regeneration were achieved in callus cultures established from the leaf base explants of Kaempferia galanga L. Callus induction and somatic embryogenesis at various frequencies were observed using different concentrations and combinations of growth regulators. The highest percentage of callus induction was observed on MS medium supplemented with 1.5 mg L-1 2,4-D+1.0 mg L-1 BA. After transfer on MS medium supplemented with 2.0 mg L-1 BA+0.1 mg L-1 NAA, this callus produced small globular embryos first that later developed into plantlets by further subculturing on the same medium. Plantlets were acclimatized and subsequently transferred to the field. Survival rate of the plantlets under ex vitro condition was 85%.

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