Abstract: RYMV transmission by insect vectors is considered to fully represent how RYMV disease is spread under natural field conditions. The present study aimed to use Oxya hyla, Locris rubra and Chnootriba similes vectors after acquisition of the virus to determine RYMV movement and distribution in insect body and transmission to rice cultivars. RYMV susceptible BG 90-2 was sown in 5 L plastic pots each at 0.5, 1 and 1.5 m distance from test entries and seedlings were mechanically inoculated with a highly virulent RYMV Nigerian isolate 14 days after sowing. Seven days after inoculation of BG 90-2, test entries were sown in 5-litre plastic pots and same day Oxya hyla, Locris rubra and Chnootriba similes vectors were introduced into the screen house to feed on RYMV infected BG 90-2. RYMV content in Oxya hyla, Locris rubra and Chnootriba similes whole body was 71.8, 44.1 and 50 and head part was 42, 44.6 and 10.1%. RYMV incidence at 0.5, 1.0 and 1.5 m vector migration distance was 14.6, 16.0 and 19.0% for Oxya hyla, 31.3, 35.2 and 39.6% for Locris rubra and 13.7, 16.2 and 19.9% for Chnootriba similes. Cluster dendrogram revealed three groups (GrpA, GrpB, GrpC) of RYMV cultivar screening methods. GrpA was typical of Locris rubra, GrpB has mechanical and Oxya hyla while Chnootriba similes formed GrpC. The information reported in this study would help to better understand RYMV disease epidemic in farmers’ fields and to develop durable resistant rice varieties against the disease.