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How to cite this article
Dennis Ochola and Geoffrey Tusiime, 2011. Survey on Incidences and Severity of Rice Yellow Mottle Virus Disease in Eastern Uganda. International Journal of Plant Pathology, 2: 15-25.
DOI: 10.3923/ijpp.2011.15.25
URL: https://scialert.net/abstract/?doi=ijpp.2011.15.25
DOI: 10.3923/ijpp.2011.15.25
URL: https://scialert.net/abstract/?doi=ijpp.2011.15.25
Waswa moses Reply
HOW DID THE AUTHOR CALCULATE ANOVA
Dennis Ochola
Dear Waswa Moses,
Although not described in detail in the methodology of this study, the unbalanced ANOVA function in Genstat allowed us to fit completely general models to unbalanced data that was derived from the 49 surveyed fields in 11 district.
WASWA MOSES
Thanks alot for the reply,
Am requesting you to send me a copy of your raw data and i see how to collect my data too, because am doing the same work but on potato viruses
Dennis Ochola Reply
Dear Waswa Moses,
I will gladly look around among my stuff if I can find the data sheet I used. I recently relocated from NaCRRI to Bioversity International so during the move I misplaced a lot of stuff. In the meanwhile, please feel free to contact me using the e-mail on this very article. Best regards.
Chibuikem I. N. Unamba Reply
Please can you explain in full details how the incidence and severity was calculated. I don't fully understand
Dennis Ochola
Dear Chibuikem I. N. Unamba,
I am glad that you have read our manuscript and would like some clarifications regarding the incidence and severity. However, I would kindly request you read through the field survey section of this manuscript therein you will find the answers to your question. The severity results are scores derived from a modification of the original scale by Raymundo et al (1979)through the addition of intermediate scores i.e. 1.5 and 2.5. Incidence is a visual estimate of area of field that is symptomatic. You can divide the entire field into strata and visually estimate the percentage of each strata that displays symptoms. Then find the average of the different strata. Hope this answers your queries. Most important be conversant with the disease symptoms, for RYMV it is similar to nitrogen deficiency. Be careful. Best regards.
Norehan Arifin Reply
Hello. happy new year. May i know further about symptoms assessment and Enzyme-Linked Immunosorbent Assay (ELISA) were used as tools of disease diagnosis in this study?
Dennis Ochola
Dear Norehan Arifin,
Happy new year.
Thanks for your question above. Symptom assessment in this article involved field assessment and screen house assessment. For the field, I modified the scale by Raymundo et al. 1979 by adding intermediate scores. Assessment was conducted diagonally in each rice field on 15 plants. ELISA was important especially to prove whether the samples picked where indeed RYMV infected and not necessarily nitrogen deficiency symptoms as is common. For further reading refer to my other paper 'Pathogenicity of Rice Yellow Mottle Virus and the Potential Sources of Resistance against the Disease in Eastern Uganda'.