Abstract: The dyeing properties of roots of Berberis aristata DC (Berberidaceae) were determined by using natural and synthetic mordants. The aqueous extract obtained from roots of the plant collected by sustainable manner was used for dyeing cotton cloths and threads. The extract of stem bark of Myrica esculenta (Myricaceae) was used as natural mordant whereas SnCl2 and FeCl3 were used as synthetic mordants. The dye solution showed significant increase in percentage absorption with natural mordant by 47.05% in comparison of synthetic mordants SnCl2 and FeCl3 with absorption percentage by 46.02 and 36.73, respectively. This work concludes that roots extract of B. aristata having good dyeing properties itself and its color can be enhance by natural mordants those are safer and eco-friendly, and can be a source of new dye stuff for textile industries.