Abstract: In present study, four tomato genotype i.e., Roma, Nagina, Naqeeb and Riogrande were investigated under the saline conditions and the half strength Hoagland’s nutrient solution was provided after a regular interval of time. Results revealed that increased level of salinity significant decreased the number of leaves per plant, root and shoot fresh and dry weight, net photosynthesis rate, chlorophyll content, leaf area and number of stomata, while increase in the concentration of organic osmolytes (total free amino acids and total soluble sugars) was observed in Roma, Nagina, Naqeeb and Riogrande tomato genotypes. The calcium content in root, shoot and leaf markedly reduced in all tested tomato genotypes under saline conditions.