Abstract: Seeds of plants belonging to Solanaceae family are well known for their alkaloidal secondary metabolites. But there exist some non-alkaloidal secondary metabolites which are less explored. The objective of the present study is to review the existence of various bioactive non-alkaloidal secondary metabolites of seeds of the medicinal plant, Hyoscyamus niger, which is commonly known as henbane. The content is based on the literature collected from Chemical Abstracts and Medicinal and Aromatic Plant Abstract (MAPA). The distribution, traditional uses, secondary metabolites particularly non-alkaloids and the pharmacological activities of H. niger seeds are described in the article. The main non-alkaloidal constituents of the anticholinergic seeds include lignans, coumarinolignans, withanolides, lignanamides, glycerides, saponins, flavonoids and its glycosides. The H. niger seeds are found to possess antimicrobial, antidiarrhoeal, antispasmodic, hypotensive, anticonvulsant, antiinflammatory, analgesic and antipyretic activities.