Abstract: Antioxidant phytochemicals such as Vitamin E and Vitamin C contents were estimated in fresh samples of three medicinal plants using a reverse-phase HPLC system. Maximum Vitamin E content (28.14 mg/100 g) was found in the leaves of Osmanthus fragrans and maximum Vitamin C content (746.79 mg/100 g) was found in the leaves of Melia azedarach. The range of Vitamin E and C in three medicinal plant was 3.53-28.14 mg/100 g and 183.13-746.79 mg/100 g, respectively.