Abstract: Nanotechnology can be defined as a research for the design, synthesis and manipulation of structure of particles with dimension smaller than 100 nm. Nanotechnology emerges from the physical, chemical, biological and engineering sciences where novel techniques are being developed to probe and manipulate single atoms and molecules. The biomimetic approaches of silver nanoparticles were reduced by a simple and eco-friendly process. The advantage of using plants for the synthesis of nanoparticles is that they are easily available, safe to handle and possess a broad variability of metabolites that may aid in reduction. We report a biomolecules hosting, rapid, environmentally benign, bio-degradable, non-toxic and green synthesis of silver nanoparticles by using Piper betle leaf broth as a reducing and stabilizing agent. The sizes of synthesized silver nanoparticles were formed on the treatment of aqueous AgNO3 solution with Piper betle broth, in the range of 3-37 nm. A UV visible spectrum of the aqueous medium containing silver ions demonstrated a peak at 440 nm corresponding to the surface plasmon resonance of silver nanoparticles; An XRD analysis reveals the crystalline nature of silver nanoparticles. The FTIR spectrum suggests that the proteins act as capping agents around the nanoparticles. The size and shape of the nanoparticles were employed by the Transmission Electron Microscope (TEM).