Abstract: Background and Objective: Chronic anemia is the main cause of death of sickle cell patients. The way to manage their anemia is the stimulating of erythropoiesis by daily folate supplementation with its side effects. This study was aimed to evaluate in vitro antihemolytic and in vivo erythropoietic properties of Morinda lucida leaves hydroalcoholic extract (MLHE). Materials and Methods: The hemolysis was induced by a range of hypotonic solutions of NaCl in the absence and presence of 1.5 and 3 g L–1 of MLHE. The toxicity of 28 days was also evaluated for MLHE at the doses of 250, 500 and 1000 mg kg–1. Results: MLHE at 1.5 and 3 mg mL–1 reduced, respectively 14.7% (p<0.01) and 23.2% (p<0.0001) this hemolysis. On other hand, three days after the anemia induction by phenylhydrazine administration, the reticulocyte rate had increased from 4.6% of red blood cells to 17.6% for the control versus 23.1% (p<0.02) in rats treated with 500 mg kg–1 of MLHE. After 28 days of MLHE oral administration (250, 500 and 1000 mg kg–1), hematology, nephrology and hepatic parameters showed no significant difference between the control groups and the treated rats. Only the platelets and white blood cells showed significant increasing but this is in the rat standard blood cell counts. Conclusion: The MLHE have anti-anemia properties by increasing the erythropoiesis and osmotic resistance of the red blood cells. Indeed, the results showed that it can admit that MLHE is not toxic.