Abstract: Gloriosa superba is a medicinal climber, its seeds and tubers contain valuable alkaloids viz., colchicine and colchicoside as the major constituents, which are used to treat gout and rheumatism. Eighteen accessions of Gloriosa superba collected from various geographical regions of the important Gloriosa growing states viz., Tamil Nadu and Andhra Pradesh were evaluated for total sugar, starch, protein, total phenol, peroxidase, catalase and poly phenol oxidase at Horticulture College and Research Institute, Tamil Nadu Agricultural University, Coimbatore to assess its suitability for seed yield as well as tolerance to leaf blight. The accessions exhibited remarkable variations for all the traits studied. The maximum peroxidase, catalase and minimum poly phenol oxidase were observed in GS 15 while GS 12, recorded the highest total sugar content. The accession GS 15 excelled in the accumulation of starch, soluble protein and total phenol in tuber and thus can be utilized for extraction of colchicine and colchicoside.