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In vitro Micropropagation using Corm Bud Explants: An Endangered Medicinal Plant of Gloriosa superba L. |
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A. Arumugam and K. Gopinath |
Abstract:
An efficient protocol was developed in vitro micropropagation of Gloriosa superba by using corm bud explant. MS basal medium supplemented with different concentration and combination of 2,4-D (2,4-dichlorophenoxy acetic acid), IAA (Indole-3-acetic acid), BAP (6-benzyl aminopurine), GA3 (Gibberellic acid), Zen (Zeatin), NAA (α-naphthaleneacetic acid), AC (Activated Charcoals) and CW (Coconut Water) were used. The 98.30±0.84% of yellowish Callus initiation was observed in MS media with (2, 4-D 1.0 mg L-1, IAA 0.5 mg L-1) after 4 week culture. This corm bud callus transferred to the shoots initiation medium. The maximum number (94.00±2.92) of multiple shoots was obtained on half strength of MS medium with (Kn (Kinetin) 1.0+BAP1.5+20% CW). The shoot lets transferred to the roots initiation medium. The 96.20±2.59% of multiple roots was obtained at the concentration of MS medium with BAP(8.0)+GA3(1.0)+Zen(0.5)+NAA(1.0)+2 g L-1 AC. In other case without addition of activated charcoal in the MS medium, only 92.20±1.92% of root initiation was occurred, 16% of root induction depends on the addition of activated charcoal present in the MS medium. The rooted plantlets were transferred into small plastic nursery tray which was containing vermi compost, sand and red soil in the ratio of 1:2:2 and kept in a mist house. After acclimatization in the mist house for 2-months, the regenerated plantlets were hardened in the greenhouse and successfully transferred into soil which shows 90% survival rate. This new protocol was standardized for easy mass propagation of such an endangered medicinal plant G. superba by using corm bud explants.
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How to cite this article:
A. Arumugam and K. Gopinath, 2012. In vitro Micropropagation using Corm Bud Explants: An Endangered Medicinal Plant of Gloriosa superba L.. Asian Journal of Biotechnology, 4: 120-128.
DOI: 10.3923/ajbkr.2012.120.128
URL: https://scialert.net/abstract/?doi=ajbkr.2012.120.128
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