Abstract: The Total Phenolic Content (TPC) and antioxidant activity of fresh and dried materials of Enicostemma littorale Blume were evaluated using the Folin-ciocalteau method, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and Ferric Reducing Antioxidant Power (FRAP) assays. Different drying treatments especially, microwave treated plant material led to significant reduction (p≤0.05) in antioxidant properties of E. littorale in methanolic extracts as compared to that of the boiling water extracts, which appeared to exhibit significantly stronger antioxidant potentials (p≤0.05) even in dried plant materials due to greater solubility of compounds, breakdown of cellular constituents as well as hydrolysis of tannins. A strong free radical scavenging activity in the chosen plant material suggests that it has great potential in the food industry as functional food ingredient.