Abstract: The hydroalcoholic extract of whole plant of Phyllanthus amarus Linn showed significant increase in activity of insulin at 200 mg kg-1 dose in regenerative hepatocytes against alcohol induced liver cell injury in partially hepatectomised albino rats. The blood sample were collected from the abdominal aorta and the serum insulin estimated by radio immuno assay and regenerative capacity measured by thymidine kinase assay by 3H thymidine incorporation into hepatic DNA which showed that Phyllanthus amarus Linn has a potential role in insulin action during liver cell regeneration.