Abstract: Malignant gliomas, the most common subtype of primary brain tumors, are aggressive, highly invasive and neurologically destructive tumors, considered being the deadliest of human cancers. As an attempt to understand the biology of glial tumor, a study on macromolecules like proteins, matrix metalloproteinases, lipids antioxidants and Deoxyribonucleic acid, in the blood of glioma patients was made. Biochemical assessment of significant pathophysiological enzymes, antioxidants and marker enzymes was performed. MMP expression was determined using gelatin zymography. Karyotyping analysis was done to determine chromosomal aberrations. A marked rise was observed in the proteins and lipids of glioma patients as compared to the normal cases. The antioxidant status of the patients was found to be lowered. Karyotypic analysis of the peripheral blood chromosomes presented various chromosomal aberrations in glioma patients. The biochemical parameters were significantly increased in the patient population (p<0.01, p<0.001) when compared to those of normal. Zymographic analysis showed the presence of MMP-2 and MMP-9 in the patient sample. Karyotypic investigation showed alterations in the chromosomal pattern of the glioma patients. The study provides baseline information on the biochemical alterations in the blood of glioma patients which can be further exploited for detailed investigations.