Submit Manuscript

Asian Journal of Biochemistry

Year: 2011 | Volume: 6 | Issue: 1 | Page No.: 104-112
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend

Total views 291

Authors

Jeeva Jothi Dhanasekaran

Mathangi Ganapathy

Keywords

Short Communication

Hepatoprotective Effect of Cassia auriculata L. Leaf Extract on Carbon Tetrachloride Intoxicated Liver Damage in Wister Albino Rats

Jeeva Jothi Dhanasekaran and Mathangi Ganapathy
Cassia auriculata L. belongs to Fabaceae and is widely distributed in India. It is used in traditional medicine, typically for skin disease, as a purgative, laxative, antihelminthic, antidiabetic and antioxidant from the ancient period. There have been reports of antidiabetic and medicinal properties of C. auriculata dried leaf and flowers. The present investigation was done to find whether the methanolic extract of Cassia auriculata leaf had hepatoprotective effect against carbon tetrachloride induced liver damage in Wister albino rats and to estimate the total antioxidant content, total phenolic content and total flavanoids of C. auriculata methanolic leaf extract. The methanol extract was used to treat the carbon tetra chloride induced liver damage on Wister albino rats for 60 days. In vitro antioxidant activity was studied using ABTS+ free radical scavenging method. The total content of phenolic compounds and flavanoids was also estimated by spectrophotometric method. The in vitro cytotoxicity activity was conducted on HepG2 cell line at increasing concentration and the apoptotic activity was determined. The animals were treated at 600 mg kg-1 b.wt. The blood serum was used for liver function test. Serum Lactate Dehydrogenase (LDH), Serum Glutamate Oxaloacetate Transaminase (SGOT), Serum Glutamate Pyruvate Transaminase (SGPT) and γ-Glutamyl Transpeptidase (GGPT), Lipid Peroxidation (LPO) and liver tissue total protein were (p<0.001) significantly decreased in post treated animals. The non enzymatic antioxidant Reduced glutathione (GSH), Vitamin C (Vit C), Vitamin E (Vit E) and enzymatic antioxidant Glutathione Peroxidase (GPx), Glutathione-s-Transferase (GST), Superoxide Dismutase (SOD) and Catalase (CAT) were increased significantly in plant extract post treated group. The histopathological studies showed fine revertible changes in CAME treated experimental animal liver tissue section.
PDF Fulltext XML References Citation

Related Articles

Anticancer and Toxic Effects of Curry Leaf (Murraya koenigii) Extracts
Cytotoxic Effects of Multiple N-nitrosamines in Human Liver Cell Line Hep2G: Possible Mechanisms of Action
Antioxidant and Anti-adhesive Activity of Some Common Lichens
Synergistic Antibacterial Activity of Four Medicinal Plants Collected from Dharapuram Taluk of Tiruppur District, South India
Phytopharmacology of Antiophidian Botanicals: A Review
Pharmacological Actions of Cassia auriculata L. and Cissus quadrangularis Wall.: A Short Review
Antidiabetic and Hypolipidemic Effect of Cassia auriculata in Alloxan Induced Diabetic Rats

Comments

abhishek raj Reply

really very knowledgeable article. thank you sir

Leave a Comment

Your email address will not be published. Required fields are marked *