Abstract: The aim of the present study was to evaluate the effects of dose-dependent of zinc chloride on short-term and long-term memory in a shuttle box. Young Wistar rats (94±10 g) (age 27-30 days) consumed zinc chloride drinking water in five different doses (20, 30, 50, 70 and 100 mg kg-1day-1) for two weeks by gavage. After 14 days on experimental diets, a shuttle box used to test short- and long-term memory. Two criteria considering for behavioral test, including latency in entering dark chamber and time spent in the dark chamber. This experiment shows that after 2 weeks oral administration of ZnCl2 with (20, 30 and 50 mg kg-1 day-1) doses, the rat’s working (short-term) has been improved (p<0.05). Whereas ZnCl2 with 30 mg kg-1 day-1 dose has been more effected than other doses (p<0.001). But rat which received ZnCl2 with 100 mg kg-1 day-1, has been shown significant impairment in working memory (p<0.05) and there was no significant difference in reference (long-term) memory for any of groups. In general, this study has demonstrated that zinc chloride consumption with 30 mg kg-1 day-1 dose for two weeks was more effective than other doses on short-term memory. But consumption of ZnCl2 with 100 mg kg-1 day-1 dose for two week had the negative effect on short-term memory. On the other hand, zinc supplementation did not have an effect on long-term memory.