Abstract: This study reports symbiotic and asymbiotic germination of the seeds of endangered species Dactylorhiza osmanica (KL.) Soo var. osmanica (endemic) and Spiranthes spiralis. (L.) Chevall. Mycorrhizal fungus (Fosm1-Rhizoctonia solani) that was isolated from the roots of D. osmanica var. osmanica was stimulated germination of the seeds of D. osmanica var. osmanica but caused rowing and browning of protocorms. Whereas the symbiotic association between Fosm1 and the seeds of S. spiralis was fully compatible. It was determined that Fsp. sp. (R. repens) didn’t compatible fungus for germination of the seeds of examined orchids, because of further development of protocorms was no observed. F1 (Binucleate Rhizoctonia AG A) was established successfully symbiotic association with the seeds of both D. osmanica var. osmanica and S. spiralis and it was stimulated germination and further development of these orchids protocorms. It was determined germination of the seeds of D. osmanica (K.I) Soo’ var. osmanica and S. spiralis (L.) Chevall in two asymbiotic culture media and the effect of inorganic nitrogen on the germination and protocorm development of this orchids seeds. Inorganic nitrogen was enhanced the germination and development of S. spiralis seeds in the Van Waes Deberg culture medium but the rate of germination of the seeds of D. osmanica var. osmanica were reduced. The germination and protocorm development were reduced in Knudson C culture medium.