Submit Manuscript

Trends in Horticultural Research

Year: 2012 | Volume: 2 | Issue: 2 | Page No.: 28-37
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend

Total views 443

Search

Authors

Potshangbam Nongdam

Nirmala Chongtham

Keywords

Research Article

In vitro Seed Germination and Mass Propagation of Cymbidium dayanum Reichb.: An Important Ornamental Orchid of North-East India

Potshangbam Nongdam and Nirmala Chongtham
Cymbidium dayanum Reichb., an epiphytic orchid is one of the popular orchids in cut flower market due to its attractive white flower with distinctive red strips. Because of unrestricted exploitation of this useful ornamental plant, its population is fast receding from the natural habitat. Effective conservation methods are required to protect this beautiful orchid from extinction. The present study was carried out to develop efficient in vitro regeneration protocols for C. dayanum using seeds as explant. High seed germination response was reported in Mitra (M) and Murashige and Skoog (MS) culture media devoid of any growth regulators when 0.2% Activated Charcoal (AC) was present. Addition of 1-Naphthyl Acetic Acid (NAA) at 0.7 mg L-1 in Mitra medium produced maximum seed germination (94.58%) and high root proliferation. MS medium enriched with 1.2 mg L-1 of NAA and 0.2% AC produced highest rooting (5.32±0.35). Incorporation of 6-benzyl Amino Purine (BAP) either alone or with Indole-3- Butyric Acid (IBA) in culture media produced very poor seed germination. The few germinated seeds gave rise to small underdeveloped protocorms which subsequently died after turning brown in color on further subculturing. Seed germination improved immensely and so did the culture growth when AC was added in the medium indicating its beneficial effect in plantlet development in vitro. Highest shooting (5.91±0.96) was observed in Mitra medium fortified with 1.2 mg L-1 of BAP and 0.2% AC.
PDF Fulltext XML References Citation

Related Articles

Establishment of Efficient in vitro Culture and Plantlet Generation of Tomato (Lycopersicon esculentum Mill.) and Development of Synthetic Seeds
Ethno-medicinal Uses of some Orchids of Nagaland, North-east India
In vitro Seed Propagation of Dendrobium (Dendrobium transparens) Orchid as Influenced by Different Media
Protocorm Regeneration, Multiple Shoot Induction and ex vitro Establishment of Cymbidium devonianum Paxt.
Production and Storage of Synthetic Seeds in Coelogyne breviscapa Lindl
In vitro Rapid Propagation of Cymbidium aloifolium (L.) Sw.:A Medicinally Important Orchid via Seed Culture
Isolation of Endophytic Fungi and Their Mycorrhizal Potential for the Tropical Epiphytic Orchids Cattleya skinneri, C. aurantiaca and Brassavola nodosa

Leave a Comment

Your email address will not be published. Required fields are marked *