Abstract: The hippocampal formation is present in all mammalian species and it is consist of the subiculum, the hippocampus and the dentate gyrus. Apart from principal neurons, the hippocampal formation contains glial cells and various types of interneurons. Glial cells in hippocampus contains the Astrocytes, microglia and oligodantrocytes. Astrocytes play a more active role in neuronal activity, including regulating ion flux currents, energy production, neurotransmitter release and synaptogenesis. Astrocytes are the only cells in the brain that contain the energy molecule glycogen. In this study 5 male albino wistar rats were used. After histological processing. The slides of the brains were stained with PTAH staining for showing the astrocytes. We showed differences in number of astrocytes in different compartment of hippocampus. There is significant difference between CA1 and CA3 and also between CA2 and CA3 areas. We concluded that functional differences can due to structural differences.