Abstract: Commercial bromelain was applied in the study to partially depolymerise chitosan to produce oligochitosan. The enzymatic reactions were determined by the liberation of reducing sugar and the reduction in viscosity. The suitable reaction conditions for reaction was found at 2 h, pH 5, 1.0% w/v substrate, 7% w/w enzyme and 50°C. At this optimum condition, bromelain was able to reduce the viscosity of chitosan to 84%. The freeze-dried oligochitosan obtained was off-white in colour. The hydrolysate is easily solubilised in water (at room temperature), up to 1% w/v, gaving a clear solution. At higher concentration, suspension started to form and marked precipitation begin to appear at a concentration of 1.5%.