Abstract: Attachment and heat resistance of Campylobacter jejuni in Enterococcus faecium biofilm were studied. E. faecium biofilm were incubated with 107 cfu mL-1 C. jejuni for 4.5 h at 23 ° C under atmospheric conditions or 12 h at 42 ° C under microaerophilic conditions. The coupons were heat treated at 56 and 63 ° C for 30, 60 and 120 sec. In addition, C. jejuni in E. faecium biofilm was incubated for up to 4 days at 23 ° C under atmospheric conditions in 50% trypticase soy broth for survival study. Hydrophobicity of C. jejuni planktonic cells, E. faecium planktonic and biofilm cells, as determined by salt aggregation test and microbial adhesion to hydrocarbon test using hexadecane were determined. D-value of C. jejuni planktonic cells at 56 and 63 ° C were 46 and 12 sec, respectively. D-value of E. faecium planktonic cells at 56 and 63 ° C were 117 and 80 sec, respectively. The presence of E. faecium biofilm reduced the lethal effect of heat on C. jejuni cells when heated at 56 and 63 ° C. C. jejuni formed biofilm on stainless steel when grown at 42 ° C under microaerophilic conditions for 12 h but the biofilm did not survive the heat treatments nor did C. jejuni cells in E. faecium biofilm. C. jejuni in biofilm persisted under atmospheric condition at 23 ° C for up to 2 days while C. jejuni attached on stainless steel without biofilm could not be recovered after two days of incubation. After forming E. faecium biofilm, became more hydrophobic than its planktonic cells. This may attract C. jejuni cells to attach on the biofilm as C. jejuni was found to be more hydrophobic than E. faecium planktonic cells.