Abstract: As rapid microbiological reports of foods and food related products for E. coli bacteria presence are of prime importance. Rapid assays for E.coli were developed by using the compound 4-methyleumbelliferyl glucuronide (MUG), which is hydrolyzed by glucuronidase (GUD) to yield a fluorogenic product. The production of glucuronidase was limited to strains of E.coli in the family of Enterobacteriaceae. For rapid confirmation of the presence of E.coli in most probable number tubes (MPN), MUG was incorporated into lauryl tryptose broth at a final concentration of 100 μg ml-1. Results of both the presumptive test (gas formation) and the confirmed test (fluorescence) for E.coli were obtained from tap water and raw milk samples after incubation of 6-24 hours at 450C, Approximately 94% of the tubes showing both gas production and fluorescence contained fecal coliforms (they were positive in EC broth incubated at 450C). Few false positive reactions were observed. The lauryl tryptose broth MUG most probable numbers assay was superior to conventional study for E. coli detection. The fluorogenic assay was sensitive and rapid but conventional study was time consuming and laborious.