Abstract: Large-scale micropropagation has been successfully achieved in Annona squamosa L. from nodal explants. Several media with different hormones and culture conditions were tried in standardization of in vitro propagation of this plant. Effect of various surface sterilants in eliminating the contamination from the explants collected from the field has been worked out. Activated charcoal has a profound effect in preventing the polyphenol leaching in cultures. BAP, Kinetin, IAA, IBA and NAA were used either alone or in combination to induce shoot buds. Explants produced shoot buds in 7-14 days. Effect of hormones on rhizogenesis was studied. IBA was found to be more effective in root induction. True to type plantlets were obtained from the explants from elite trees. Present protocol is rapid, reproducible and less cumbersome over the conventional methods.