Abstract: Rice α-amylase and glucoamylase produced by S. cerevisiae YKU 107 and YKU 131, respectively were partial purified using anion exchange chromatography and characterised. The optimum active conditions for -amylase was at pH 5.0 and temperature of 50°C. It was capable in hydrolysing more than 85% of potato, rice, cassava and sago starch, however, it could only hydrolysed 64% of corn starch. Glucoamylase was optimally active at pH 5.5 and temperature of 55°C. Glucoamylase could hydrolyse more than 70% of cassava, sago and potato starch, it also hydrolysed more than 65% of corn and rice starch. Both of the enzymes were not severely affected by the changes of ionic strength of acetate buffer at molarity lower than 0.20 M.