Abstract: Anatomical responses of roots in trifoliate orange budded with satsuma mandarin (Citrus unshiu Marc. cv. Okitsu Wase) to aerated and stagnant culture solutions were investigated. Roots, under aerated condition, had sharply pointed tips and showed intact anatomical features having a few starch grains on the rootcap and cortical cells. The cortical cells were smaller close to the root tip and gradually became elongated towards the base. All these characteristics maintained the same level even up to 10 days of observations. On the contrary, under stagnant condition, secretion of mucilaginous substances and root tip injuries were found on the 3rd day of induction which became more conspicuous on the 5th. These injuries resulted the severe sloughing of cells and starch grain degradation in the rootcap. Eight day induced roots displayed more distinct root tip injuries and discharging of mucilaginous substances. On the 10th day, air spacing between the cortical cells were conspicuous. Epidermal injuries were also evidenced sporadically on the 5th day and became more severe subsequently. Degradation of starch grains gradually completed in the cortical cells along the exposure time. The enlargement of periblemic cells were noticed which gradually resulted larger cells close to the root apex on longer exposures.