Abstract: Effects of leaf age on ion uptake (Na+, K+ and CI‾) and K+/Na+ ratio were investigated using the three S1 wheat selections Alex-1, KRL-24 and Khar-1. Na+ and CI‾ contents were found to be increasing in the older leaves, but K+ content and K+/Na+ ratio were found to be decreasing in the younger leaves. However, it is suggested from the results that sampling should be done when leaves are fully expanded. Khar-1 was found to be intermediate between Alex-1 and KRL-24 in ion uptake and K+/Na+ ratio at three out of the four sampling dates. The considerable variation in Na+, K+, Cl‾ uptake and K+/Na+ ratio between sampling dates suggests that these traits might be less useful as selection criteria.