Abstract: The purpose of this research was to test the biotoxicity of Ochratoxin in weaning male albino rat’s kidney and to investigate the biosafty of Ajwa dates for prevention and protection against pathological Ochratoxin alterations. Animals were gavage administrated and divided into four groups: first group received (sodium bicarbonate), second group received (289 μg OTA kg-1 B.W day-1), third group received (1 mg Ajwa kg-1 B.W day-1) and fourth group received (289 μg OTA kg-1 B.W day-1+1 mg Ajwa kg-1 B.W day-1). Serum (creatinine-urea) levels were measured in each group at the time of tissue collection , some biopsies were fixed in 10% buffered formalin solution for light microscopy processing stained with Haematoxylin and Eosin (H and E.), Periodic Acid-Schiff (PAS) and Masson’s Trichrome (M.T.). Other biopsies were immediately collected into electron microscopy processing. After 28 days, a significant decrease in body weight, kidney weight and relative weight was detected in Ochratoxin-treated group. Also, Serum (creatinine-urea) level were elevated .The normal cyto-architecture of proximal tubules were lost exhibiting damaged bruch border, degenerated, binucleated and karyomegalic cells. The most destructed ultra-structure was the mitochondria which severely swollen with disintegrated membranes. In Ajwa Date extract-group the proximal tubules were normal, whereas in Ajwa date extract+Ochratoxin-group the severity of the lesions was significantly reduced. The present results indicate that Ajwa date have protective effects and ameliorated the lesions of Ochratoxin nepherotoxicity which might led to kidney failure.