Abstract: The current study dealt with the interaction of Garlic Homogenate (GH) with propranolol (PRO) on isoproterenol (ISO)-induced Myocardial Infarction (MI) in rats. Albino rats were treated either with GH at three different doses of 125 mg kg-1, (GH-125), 250 mg kg-1 (GH-250) and 500 mg kg-1 (GH-500) orally for 30 days or different doses of GH along with PRO (10 mg kg-1, p.o.) during the last 7 days of GH treatment. Myocardial damage was induced by administration of ISO (150 mg kg-1 body weight s.c.) for 2 consecutive days. The PRO, moderate dose of GH alone or in combination with PRO was found to ameliorate the effect of ISO on superoxide dismutase (SOD), catalase and retained the activities of the diagnostic marker enzymes such as lactate dehydrogenase (LDH) and creatine phosphokinase isoenzyme (CK-MB). Incorporation of PRO during GH treatment provided further protection to myocardium from injury. However, higher dose of GH alone or in presence of PRO failed to prevent ISO induced myocardial injury. The results of the present study indicate that mild to moderate doses of GH exerts a protective effect, whereas, high dose of GH shows toxic effect against ISO-induced MI either alone or with PRO.