Abstract: The present study was aimed to study the protective effects of honeybee propolis against the amitraz hepatotoxicity in mice. Forty-eight male Swiss albino mice of 8 weeks of age, 22 to 25 g body weight was divided into four groups. The 1st was control, the 2nd treated orally with 150 mg kg-1 propolis extract, the 3rd treated with 160 mg kg-1 amitraz and the 4th one had 160 mg kg-1 amitraz + 150 mg kg-1 propolis extract. These daily treatments lasted for 8 weeks and laboratory assays were measured weekly. Results, after mice sacrificed, histopathology and immunohistology tests were carried out. The obtained results revealed that amitraz had affected liver biochemicals concentrations, whereas propolis led to a significant decrease in these levels in treated group. But, hepatocytes of mice treated with amitraz + propolis demonstrated positive stained nuclei, by using Ki67 immunostaining, less than those of amitraz treated only. The study suggests that propolis ameliorated the recovery of hepatotoxicity of amitraz in the tested mice.