Abstract: Objective: Staurogyn (Staurogyne repens (Nees) Kuntze) is a perennial herb, widely used in aquarium landscape design. Representatives of the Staurogyne species are sources of unique glycosides in their chemical composition, which are taste stimulators. However, a method of the tissue culture of S. repens was not described in the literature until now. In this study, such a method is proposed. Materials and Methods: Node bines with axillary buds were taken as explants. Surface sterilization with mercuric chloride (HgCl2) was found to be the most effective (60% survivors of sterile explants). Solid and liquid media contained 3% sucrose and various combinations of plant hormones: Benzyladenine (BA), thidiazuron (TDZ) and indole-3-acetic acid (IAA) were applied. Results: It was shown that the intensive multiplication of shoots can be induced using a liquid Murashige-Skoog (MS) medium with the following combination of phytohormones: Benzyl adenine (2 mg L–1) and indole-3-acetic acid (0.5 mg L–1). The MS concentration of 0.2 mg L–1 was the most effective for the rhyzogenesis. The adaptation of plants was successful enough under conditions of regular liquid media changeout and under maintaining overwater air humidity of 90% when the plants were grown in emergent conditions. Conclusion: The developed tissue culture method for micropropagation of aquatic plant Staurogyne repens can used be for commercial purposes.