Abstract: Mentha piperita is a very important medicinal plant used in preparations of various food products. At present a high frequency regeneration protocol has been developed from nodal explants of Mentha piperita using Murashige and Skoog’s (MS) medium supplemented with varying combination of 0.20, 0.30, 0.40 and 0.50 mg L-1 of Naphtalene Acetic Acid (NNA) and Kinetin (KN). The highest number of shoots (3) and shoot length (9.1 cm) was obtained in medium containing 0.40 mg L-1 of NAA and 0.40 mg L-1 of KN combination. The shoot length was increased up to 5.5, 7.0, 9.1 and 8.7 cm on 30 days of culture. After 30 days, the whole regenerated plants were transferred to rooting medium. This study is found to be an efficient and effective method for the conservation of this plant.