Abstract: In vitro technique has been developed using single sprout bud explants of elite indigenous potato (Solanum tuberosum L.) cultivar (cv. Indurkani) of Bangladesh. MS basal medium supplemented with different concentrations and combinations of BA and Kinetin were used to induce shoots and roots. Shoot and root formations were found to be better in combine treatment of BA and Kn than single treatment of BA or Kn alone. The highest number of shoot were formed on MS basal medium supplemented with a combination of 3.0 mg-1 BA and 2.0 mg L-l Kn whereas 2.0 mg L-l BA alone produced only 80% shoots and 2.0 mg L-l Kn alone produced 100% shoots. The induction of multiple shoots from each bud was highest (3.0) in a combination of 2.0 mg L-l Kn and 1.0 mg L-l BA while 2.0 mg L-l BA alone produced only 1.37 shoots. The maximum number of rooting was obtained with 0.5 mg L-l Kn+2.0 mg L-l BA. The highest shoot growth rate (10.5 mm/week) was observed on MS basal medium supplemented with 0.2 mg L-l BA and 2.0 mg L-l Kn. The rooted plantlets were hardened and successfully established in the soil.