Abstract: Whole body meats of mollusc, Trochus tentorium was extracted with four solvents such as acetone, ethyl acetate, dichloromethane and methanol. Maximum antibacterial inhibition zone was exhibited from acetone crude extract of T. tentorium against human pathogen (Streptococcus pneumoniae 8 mm). The 100% column purified acetone fraction of T. tentorium showed higher activity against human pathogens like Klebsiella pneumoniae (7 mm). The minimal inhibitory concentration of the 100% acetone fraction of T. tentorium was found to be lower for the pathogens, E. coli (0.08 mg), Streptococcus pnuemoniae (0.08 mg), Staphylococcus aureus (0.09 mg) and Vibrio cholerae (0.09 mg), hence 100% acetone fraction of the extract of T. tentorium was deemed as potent bioactive compounds against those pathogens.