Abstract: The aim of the present study is to investigate the effect of ethanol and the action of carnosine treatment on certain liver parameters; enzymes, total proteins and glycogen in rats. These enzymes including Krebs cycle enzyme: succinate dehydrogenase (SDH); glycolytic enzymes: lactate dehydrogenase (LDH) and its isoenzymes; glycogenolytic metabolic machineries: glycogen phosphorylase, glucose-6-phosphatase (G-6-Pase) and glycogen; hydrolytic enzymes: acid phosphatase (AP) and alkaline phosphatase (ALP); amino acid metabolic enzymes: aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and nucleic acid catabolic enzyme: 5`-nucleotidase. The chronic ethanol-intoxicated rats showed a marked disturbance in all the measured parameters, indicating the toxic effect of ethanol by inducing oxidative stress on liver tissue. Administration of carnosine ameliorated the toxic effect of ethanol by enhancement all of the measured parameters.