Abstract: In order to test the efficacy of different laboratory techniques used in diagnosis of tuberculosis, 50 Tb cases along with 50 control cases in Faisalabad area were studied, using different laboratory tests. Mantoux’s test showed no correlation with hematological parameters. Acid fast test (AFB-Test) was routinely used in labs, but there was still chances of contamination of sputum sample with a typical mycobacteria, so increasing the chances of error in the results. Chest radiograph was also a specific technique to some extent but in case of small children and other lungs diseases, it became unreliable and confusing test. When a comparative evaluation was made between different diagnostic tests, polymerase chain reaction (PCR) proved to be a very sensitive, highly specific, qualitative and quantitative technique for the confirmation of pulmonary tuberculosis. However, there was no correlation of PCR results with other hematological findings. Hematological studies showed significantly high level of erythrocyte sedimentation rate (ESR) in Tb cases (64.3 ± 38.2) (P<0.00) than in control cases (16.57 ± 5.26) were significantly increased (72.5 ± 39.9). Hemoglobin level (Hb) of Tb patients was significantly decreased (8.84 ± 2.33) than in control cases (11.8 ± 1.65). Total Leucocyte count (TLC) in Tb patients was significantly (P<0.000) higher (8882 ± 1650) as compared to control cases (62134 ± 1161.68). Differential leucocyte count (DLC) analysis of Tb cases in comparison with control cases showed significantly high (P<0.000), neutrophil count in Tb cases (69.4 ± 4.69) as compared to control cases (60.04 ± 5.15). Lymphocyte count in Tb cases was significantly decreased (P<0.000) (24.55 ± 3.64) than in control cases (32.39 ± 4.63) and eosinophil count in Tb cases was (2.16 ± 1.34) and control cases (3.2 ± 1.55) at probability level of (0.0004). Monocytes and basophil count was non-significantly different (P<0.1127; P<1.00) in Tb cases (3.7 ± 1.72, 0.08 ± 0.27) from control cases (4.38 ± 2.55, 0.08 ± 0.27). It may be concluded that PCR test is reliable, which may be employed in routine testing of Tb cases to safeguard against misdiagnosis.